2018-08-16 09:40:172025-05-28 04:59:23
description
RNase J1, important for the degradation of RNA fragments containing transcription terminators
RNase J1, important for the degradation of RNA fragments containing transcription terminators, resolves stalled transcription complexes
locus
BSU14530
BSU_14530
geneLength
1665
1668
outlinks
bsu
BSU14530
BSU_14530
Gene
Coordinates
1,523,118 → 1,524,785
1,523,118 1,524,785
The protein
Catalyzed reaction/ biological activity
endonuclease and 5'-3' exonuclease
degrades [[protein|SR5]] and ''[[gene|gapA]]'' RNAs [pubmed|27449348]
degrades [[gene|yflS ]]mRNA [pubmed|28436820]
endonuclease and 5'-3' exonuclease
degrades [[protein|SR5]] and ''[[gene|gapA]]'' RNAs [pubmed|27449348]
degrades [[gene|yflS ]]mRNA [pubmed|28436820]
resolves stalled transcription complexes by a “torpedo” mechanism, whereby RNase J1 degrades the nascent RNA and causes the transcription complex to disassemble upon collision with [SW|RNA polymerase] [pubmed|31840842]
The protein
Protein family
RNase J subfamily (according to Swiss-Prot)
[SW|metallo-beta-lactamase superfamily] (according to UniProt)
The protein
Effectors of protein activity
initeraction with [[protein|GapA]]-[[protein|Sr1]] stimulates activity [Pubmed|27449348]
initeraction with [[protein|GapA]]-[[protein|Sr1]] stimulates activity [Pubmed|27449348]
a NAD-cap stabilizes RNA against exonucleolytic attack by RNase J1 [pubmed|30110644]
The protein
Additional information
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
required for [[gene|thrS]] RNA processing, involved in maturation of the 5’-end of the 16S rRNA
subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]
required for [[gene|thrS]] RNA processing, involved in maturation of the 5-end of the 16S rRNA
Biological materials
Mutant
GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer] lab
GP2506 (Δ[[gene|rnjA]]::''spc''), available in [SW|Jörg Stülke]'s lab
GP41 (''rnjA'' under control of p(xyl)), available in [SW|Jörg Stülke]'s lab
SSB342 (''rnjA'' under pspac), cat, available in [SW|Harald Putzer]'s lab
GP2506 ([[gene|rnjA]]::''spc''), available in [SW|Jörg Stülke]'s lab
Biological materials
Expression vector
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab
pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
Biological materials
lacZ fusion
pGP418 (in [[protein|pAC7]]), available in [SW|Jörg Stülke]'s lab
pGP418 (in [SW|pAC7]), available in [SW|Jörg Stülke]'s lab
Labs working on this gene/protein
[SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]
References
Reviews
References
Original publications
Biological materials
Expression vectors
for chromosomal expression of RNase J1-Strep (spc): GP1034, available in [SW|Jörg Stülke]'s lab
for chromosomal expression of RNase J1-Strep (cat): GP1042, available in [SW|Jörg Stülke]'s lab
pGP1441: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab
labs
[SW|Harald Putzer], IBPC Paris, France [http://www.ibpc.fr/UPR9073/putzer/recherches_harald.htm Homepage]
[SW|David Bechhofer], Mount Sinai School, New York, USA [http://www.mountsinai.org/Research/Centers Laboratories and Programs/Bechhofer Laboratory?citype=Physician&ciid=Bechhofer David H 1255565 Homepage]
[SW|Ciaran Condon], IBPC, Paris, France [http://www.ibpc.fr/UPR9073/condon/index_en.html Homepage]